PELLAM JOURNAL OF SCIENCE

Camel in Chemistry II

Camel Placenta Extracts

(References in the author’s "75 Years Chemistry - Re-Reading", Part I to V-B (2011-2015), ISBN 978-1-828292)

E-mail information@bibliothequeworldwide.com

John Pellam John Pellam John Pellam John Pellam John Pellam John Pellam John Pellam John Pellam John Pellam John Pellam The BWW Society The Bibliotheque World Wide Society The Institute for Positive Global Solutions Pellam Journal of Science

by Inst. Gov. Dr. Dr. Randolph Riemschneider

Freie Universität Berlin, Germany,

and Universidade Federal de Santa Maria, Brazil

As mentioned already in I: Camel Placenta Extract can substitute Bovine Placenta Extract in future. Here some experimental details about camel organ extracts: Tab. 1, 2, 3, and 4

Table 1: Activity tests and quality standard of CAMEL-extracts and BOVINE-Placenta extract

Table 2: Working prescription of camel placenta extract from deep frozen camel placenta glands: CAMEL Extract I

Table 3: Working prescription of a synthetic camel extract: CAMEL Extract II

Table 4: PADBERG tests

Table 1: Activity tests and quality standard of CAMEL-extracts and BOVINE-Placenta extract[i] sstandardized protein free

Activity tests*

CAMEL I natural

Tab 1

CAMEL II synthetic

Tab 2

BOVINE natural

Metabolic activity

(WARBURG-factor)

2,0

> 2,2

1,7 – 2,0

Growth test

shortening of metamorphosis

of tadpoles Xenopus laevis DAUDIN in days

5 – 6

6 - 7

4 - 6

Wound healing

skin tension after 20 days

diff. between exper. and control group

240

235

PADBERG-test

65 (80)***

70 (85)***

Corneometer test

Humidity maintaining capacity

Data after standardization

Standardized proteinfree** Placenta-Extracts

CAMEL I, CAMEL II, BOVINE

dry substance

amino acids (ninhydrin)

peptides (biuret)

nucleo acid components

hormones

heavy metals

sterility

6,7 – 6,9

0,40 – 0,75 %

0,04 – 0,07 %

positive

negative

< 20 ppm

germfree

* Test methods described in (3)

** The author realized since 1965 the successful application of several protein free [and enzyme free] cosmetic additives like n-PFE, K-PFE, Cellryl + k-PFE in Evangyl, Omnithymus and most recently swine P.E.B.D.

*** Control experiment data in brackets.

Table 2: Working prescription of camel placenta extract from deep frozen camel placenta glands: CAMEL Extract I (natural)

First, disinfect the apparatus to be used.

Step 1: 20kg deep-frozen placenta glands supplied with a certificate that they are free of epidemics and diseases[ii] are thawed in big tubs under running cold water (duration approx. 8 to 10 hours). If a total of 100 kg of material is supplied, this means repeating the procedure 4 to 5 times.

Step 2: The thawing or thawed glands are then placed into a washing machine (horizontal cylinder) and washed with the continuous addition of water until they are completely free of blood: duration approx. 12 hours.

Step 3: The placenta glands which are still wet and have been washed completely free of blood are comminuted to a particle size of 0.3 mm in a microniser (Fryma) and collected in V₂A jugs, immediately adding 500ml of diethyl ether and preservative per jug. This mush is stirred well to distribute the additives. Be sure to observe protective measures (Ex-Schutz), arrange for thorough ventilation, wear a protective mask, observe VbF, as diethyl ether is an A 1 liquid).

Step 4: Allow the jugs to stand for about 5 days at 0 to 4°C with frequent stirring.

Step 5: After 5 days, the placenta gland mush is separated in an explosion-proof cylinder centrifuge. This centrifuge is loaded until liquid exits from the outlet pipe. One centrifuge cycle should be about 20 minutes. The motor is then turned off and the centrifuge brought to a standstill with careful braking.

When the centrifuge has come to a standstill, lower the outlet tube and drain the centrifuged matter via a V₂A screen into V₂A jugs. (The room must be well ventilated and a protective mask needs to be worn because of the diethyl ether.) Repeat step 5 until the entire mush of placenta glands has been processed. Freeze the placenta gland residues.

Step 6: Combine all of the centrifuged material in one vessel and filter through 40 cm Seitz layer filters until clear. Start with 4 layers K 1,500 and, if required, x layers of K 300, K100, K 50 and EK.

Step 7: Adjust the pH of the filtrate to 7.5 to 7.8 with 2n NaOH and pass air through the filtrate with stirring until no trace of a diethyl ether smell is detectable.

Step 8: Filter the total mass through EK filters.

Step 9: Adjust the pH to 3.4 with citric acid and ventilate with oxygen over 8 hours; if need be, add 1 g of activated carbon per 1 l of centrifuged material to clarify the filtrate.

Step 10: After a test has been carried out to ascertain that the filtrate is free of protein (sulfosalicylic acid test), filtration is carried out through EK filters and subsequently millipore filters (sterilised), pore width 0.2 µm, into plastic canisters (previously vapour-sterilised) under a laminar flow work bench.

Table 3: Working prescription of a synthetic camel extract: CAMEL Extract II

The following components calculated to yield a final volume of 1 litre were dissolved with stirring under sterile conditions in one volume of bi-distilled water which corresponds to 3/10 to 4/10 of the final volume to be adjusted; the pH value should be kept between 6.0 and 7.4.

L-Aminosäuren und Derivate: Glycin, Glutaminsäure je 0,3-0,4g, a-Alanin, Asparaginsäure, Hydroxyprolin, Prolin, Serin, Lysin, Arginin je 0,2-0,4g, Histidin, Ornithin, Asparagin, Valin, Tyrosin, DL-Threonin. Phenylalanin, Leucin je 0,03-0,06g, Threonin, Tryptophan, Methionin ß-Alanin je 0,01-0,03g, Isoleucin, Cystein, Kreatinin, Hippursäure je <0,002g, Urea 0,5-0,9g.

Oligopeptide mit 3-10 Aminosäuren + pflanzl.Peptone bzw. Peptonhydrolysate zus.3,0-5,0g.

Nucleinsäurekomponenten + Derivate: Adenin, Adenosin, Cytidin, Guanin, Cytosin, Uracil, Guanosin, Uridin, Hypoxanthin, Xanthin, cycl.AMP, Adenosinmonophosphat je 0,01-0,03g, Inosin 0,08g, Harnsäure, Orotsäure 0,002g. KHe: Glucose, Mannit, Sorbit je 0.1-0,3g,Säuren:

Bernsteinsre 0,1-0,3g, Äpfelsre 0,01g, Citronensre. 0,2g, Alkohole: Ethanol 1,0-3,0ml, Benzylalkohol 0,3ml, Glycerin 0,4-0,5ml; Vitamine: Pyridoxol-HCl 0,5-0,7g, Biotin 0,1g, Thiamin-chlorid, Ca-pantothenat, Tocopherolsuccinat je <0,002g, myo-Inosit Nicotinsreamid je <0,03g. Mineralsalze: Mg-sulfat, Mg-aspartat, NaH₂PO₄-H₂O je <0,07g, Zn-acetat: 0,1g, Co-, Mn-gluconat je 0,005g. Sonstige Zusätze : N-Methylglucamin < 0,5g, Na-lactat 1,0-2,0g., Konservierungsmittel, gegebenenfalls ohne.

Durchführung: Zunächst werden die Aminosäuren Glu,Asp,Val,Tyr,Phe, Isoleu, Leu in 2 N-NaOH vorgelöst. Dann erfolgte Zugabe der 3 Carbonsäuren. Nach Einstellung des pH-Wertes auf 6,4 wurden dem Ansatz die folgenden Aminosäuren Ala,His, Gly, Orn, Asparagin, Thre, Hydroxyprolin, Kreatin, Met,Try, Pro,Ser,Arg-HCl, Lys-HCl, Hippuräsure, Harnrstoff, hinzu-gegeben. Die Nucleinsäurekomponenten Xanthin u. Guanin wurden in 3 H NaOH bzw. 3N HCl vorgelöst und dann weitgehend neutralisiert. pH der erhaltenen Teillösung I auf pH 6,4 einstellen. Unter Rühren dann erfolgte die Zugabe von Teillösung II, die wie folgt hergestellt wurden: Eine aus Mais, Soja und „partiell dehydratisierter Hefe“ im Gewichtsverhältnis 1:1:10 gewonnene Peptonmenge von 4,0g wurde zur Desodorierung und Entfärbung in einer 40fachen Menge bidest.H₂O unter Rühren gelöst und über Nacht mit Aktivkohle behandelt.

Die so erhaltene Peptonlösung wurde nach Abnutschen und Sterilfiltration durch ein 0,2um Filter (Millipore) der Teillösung I zugegeben. Dann wurden dem Ansatz die synthetischen Peptidfragmente Gly-His-Lys, Gly-His-Pro und Glutathion in einer Gesamtkonzentration von 0,1mg/L zugegeben. Anschließend den Ansatz auf 1L von pH 6,4 bringen. Zur Abfüllung 0,2um Millipore-Filtration.-- pH 6,4, Feststoffgehalt 1,4%, N 0,1% , WARBURG-Faktor >1,8; hormonfrei, BSE-frei (gemäß Herkunft aus einem BSE-freien Land). Schwermetalle: <20ppm, Sterilität: absolut keimfrei, pyrogenfrei. dermatologische teste: ohne Reizwirkung.

All CAMEL- and BOVINE-Extracts passed well all the following toxicological and allergological tests according to the Staff of Division of pharmacology FDA: Appraisal of Safety of chemicals, drugs, and cosmetics 1959. The toxicological tests based on lab animals were demanded by Asian customers to get import licence of the extracts and to guarantee safety of their clients (but against the conviction of the author). Screening TURNER, epicutaneous sensibilisation-tests: guinea pig BÜHLER, eye- and skin-irritation test: rabbit, and also DRAIZE-, AMES-, and PATCH-test.

Table 4: PADBERG tests CAMEL I- Extract compared with BOVINE-Extract as cosmetic additives *

Age of the test person	WO-creme with 1%	WO-creme with 1%	PLACEBO
	CAMEL I extract	BOVINE extract
43	53,1	59,9	94,9
53	59,7	67,3	90,0
49	59,6	50,0	95,0
59	55,8	59,9	89,6
48	56,9	53,6	94,1
42	63,9	70,5	77,0
51	61,0	62,1	89,1
56	69,9	67,5	80,0
60	47,1	60,0	77,6
50	48,4	55,1	79,5
51	49,9	53,0	83,3
60	45,1	66,0	78,9

^) Test method described in EP 0 552 516 B1 in Part V-B*, p 243: Hautrauhigkeit (%) = x 100

Experiments carried out in Santa Maria, UFSM and in Curitiba, BRASTONE.

Later in Japan, Yamakawa and Company, Ltd., Tokyo: Control analysis of ordered standardized CAMEL Placenta Extract - according to table 1.

Country of origin of CAMEL Placentae glands: Australia as a BSE-free country; cf. footnote ii